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C-reactive protein detection method and kit

机译:C-反应蛋白检测方法和套件

摘要

The present invention provides a kit for detecting a full range of C-reactive protein and a method for detecting a full range of C-reactive protein based on chemiluminescence immunoassay. The kit includes R1 reagent, M reagent, R2 reagent, pre-excitation solution and excitation solution. The sample treatment solution, R1 reagent, is a 0.5M citric acid solution (pH 3.0-3.5, adjusted with disodium hydrogen phosphate dodechydrate). In addition, the present invention is a flat bottom plate type chemiluminescent plate coated with a first antibody, a sample treatment solution, a second antibody labeled with horseradish peroxidase (HRP) or alkaline phosphatase (AP), and a color developing solution. -Provides a kit for detection of a full range of reactive proteins. In addition, the present invention provides a method for detecting a full range of C-reactive proteins using the kit. Both the first antibody and the second antibody are monoclonal antibodies capable of specifically reacting with a C-reactive protein.
机译:本发明提供了一种用于检测全系列C反应蛋白的试剂盒和用于检测基于化学发光免疫测定的全系列C-反应蛋白的方法。该试剂盒包括R1试剂,M试剂,R2试剂,预激溶液和激发溶液。样品处理溶液R1试剂是0.5M柠檬酸溶液(pH3.0-3.5,用磷酸二钠DodeChudrate调整)。此外,本发明是一种覆盖有第一抗体的扁平底板型化学发光板,样品处理溶液,用辣根过氧化物酶(HRP)或碱性磷酸酶(AP)标记的第二抗体,以及显影溶液。 - 提供一种试剂盒,用于检测全系列的反应蛋白。此外,本发明提供了一种用于使用试剂盒检测全系列C-反应蛋白的方法。第一抗体和第二抗体都是能够与C反应蛋白特异性反应的单克隆抗体。

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