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METHOD FOR FULL COURSE DETECTION OF C-REACTIVE PROTEIN AND CORRESPONDING KIT

机译:全课程检测C反应蛋白和相应试剂盒的方法

摘要

The invention provides a kit for full-range detection of C-reactive protein based on chemiluminescence immunoassay and a method for full-range detection of C-reactive protein. The kit comprises an R1 reagent, an M reagent, an R2 reagent, a pre-excitation solution and an excitation solution. The R1 reagent, that is a sample treatment solution, is a 0.5M citric acid solution (pH 3.0-3.5, which is adjusted by disodium hydrogen phosphate dodecahydrate). The present invention also provides a kit for full-range detection of C-reactive protein, which comprises a flat-bottomed plate-type chemiluminescence plate coated with a first antibody, a sample treatment solution, a second antibody labeled with horseradish peroxidase (HRP) or alkaline phosphatase (AP), a color developing solution. The invention also provides a method for full-range detection of C-reactive protein using the kit. Both the first antibody and the second antibody are monoclonal antibodies that can specifically react with C-reactive protein.
机译:本发明提供了一种基于化学发光免疫测定的C反应蛋白的全系列检测试剂盒,以及C反应蛋白的全射蛋白的全射蛋白的方法。试剂盒包含R1试剂,M试剂,R2试剂,预激溶液和激发溶液。作为样品处理溶液的R1试剂是0.5M柠檬酸溶液(pH3.0-3.5,由磷酸二钠十二次水合物调节)。本发明还提供了一种用于C反应蛋白的全范围检测的试剂盒,其包括涂有第一抗体的平底板型化学发光板,样品处理溶液,用辣根过氧化物酶(HRP)标记的第二抗体或碱性磷酸酶(AP),一种颜色显影溶液。本发明还提供了一种使用试剂盒的C反应蛋白的全范围检测方法。第一抗体和第二抗体都是单克隆抗体,其可以与C反应蛋白特异性反应。

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