SET FOR DETECTING SARS-COV VIRUS BY REAL-TIME RT-PCR METHOD

摘要

FIELD: biotechnology; medicine.;SUBSTANCE: invention refers to biotechnology and medicine, namely to diagnosing infectious diseases, particularly to detecting genetic markers of coronavirus SARS-CoV-2. Kit for detection of coronavirus type SARS-CoV-2 by RT-PCR in real time includes oligonucleotide primers, which limit a fragment of RNA gene of dependent RNA polymerase, and a fluorescent labeled fluorescent (fluorescent probe) oligonucleotide having the following structure: CoV2-f (SEQ ID NO.1) tca caa ttc aga agt agg acc tg, CoV2-r (SEQ ID NO.2) agc ctc caa agg caa tag tgc, CoV2-prc (SEQ ID NO.3) R6G - tct tgc cga ata cca taa tga atc tgg caa - BHQ1.;EFFECT: present kit for detecting coronavirus type SARS-CoV-2 by RT-PCR in real time enables extending the range of available diagnostic agents.;1 cl, 1 dwg, 4 tbl