method for production of cephalosporin c from the antibiotic cephalosporin containing in addition a fermented medium

摘要

Cephalosporin C is recovered from a clarified beer containing it and co-present Cephalosporin N by lowering the pH of the clarified beer preferably adjusted to pH5-6 with acetic acid or sodium hydroxide by treatment with a cation exchange material containing strong acidic groups in hydrogen form to a value of from 2.8 to 4.0, separating the cation exchange material from the acidified medium, then passing the acidified medium through a strong anion exchange material in the form of a salt of a weak monobasic organic acid e.g. acetic acid to remove substantially all chloride and other inorganic anions therefrom and thereafter separating the Cephalosporin C from the percolate. The Cephalosporin C may be separated from the percolate by contacting it with an anion exchange material in the form of a salt of a weak monobasic organic acid e.g. acetic acid to adsorb the Cephalosporin C thereon and eluting the adsorbed Cephalosporin C therefrom with acetic or formic acid which, in the case where the anion exchange material is a weak anion exchange material is buffered by the addition of a weak base which is volatile enough to be removed by distillation in vacuo from aqueous solution at a temperature below the decomposition temperature of Cephalosporin C e.g. pyridine or ammonia. The Cephalosporin C may be precipitated from the eluate by acetone, dissolved in water and converted to its sodium salt by treatment with sodium hydroxide or a strong cation exchange resin in sodium form. The removal of chloride and other anions may be effected by a plurality of sections of strong anion exchange material. The Cephalosporin C may be separated from the percolate by adsorption on alumina or charcoal and elution therefrom. Specification 810,196 is referred to.