Isolation of coumermycin from fermentation broths

摘要

Process for separating a complex of the antibiotic coumermycin (previously called sugordomycin) from a fermentation broth contng. it. The broth obtd. by culturing Streptomyces hazeliensis NRRL 2938 (cf. Bel. 665,237) is adjusted to pH 2-5 (pref. 4), filtd. and the cells treated with an aqs. solvent system (25-75% water) contng. an org. solvent miscible with water (e.g. MeOH, EtOH, PrOH, i-PrOH, t-BuOH, Me2CO, THF, dioxan, ethyleneglycol or propyleneglycol dimethyl ether, DMSO, DMF, Me2NCOMe) at pH 6-9 (pref. 7.3-7.8), the mixt. filtered and the complex isolated from the filtrate, opt. after partial removal of solvent, by adjusting the pH to 2-5 (pref. 4) to ppt. it. An extraction rate of 90% or more can be achieved.