Procedure for the obtaining of liquid, stabilized, enzymatic preparations. (Machine-translation by Google Translate, not legally binding)

摘要

Procedure for obtaining stabilized liquid enzyme preparations, or washing and cleaning agents that contain them; characterized in that in a first stage, A. 1-30% by weight of one of the following organic, water-soluble compounds: a) aliphatic or alicyclic hydroxyalkylamines, which for each hydrocarbon residue contain a maximum of 8, preferably a maximum of 6 carbon atoms, as well as their ethers with C1-4 alcohols, b) heterocyclic compounds, optionally containing hydroxyl groups, containing a maximum of 8 carbon atoms in the molecule with an oxygen and/or nitrogen heteroatom, c) carboxy- or phosphorous-alkyl- or hydroxyalkyl-amides containing 2-8, preferably 2-6 carbon atoms in the molecule, in the form of its 50% aqueous solutions, it is neutralized, under cooling to a temperature below 4ºC, with mechanical stirring, to a pH of approximately 6.5, by dripping into said solution an aqueous solution of B. 0-30, preferably 1-30% by weight of a carboxylic or hydroxycarboxylic acid with a maximum of 6 carbon atoms, or a water-soluble, organic or inorganic salt of this acid; in a second stage, the resulting solution is optionally mixed with C. further components of the washing and cleaning agents, as well as water; in a third stage, and with stirring, an aqueous solution of D. 1-45% by weight of at least one of the following water-soluble organic compounds: d) di-to hexavalent aliphatic alcohols with a maximum of 8, preferably a maximum of 6 carbon atoms per molecule and/or polyethers of these polyvalent alcohols and/or partial ethers derived from these polyvalent alcohols, containing at least one hydroxyl group in the molecule, with monovalent C1-4 alcohols, e) carboxy- or phosphorousalkyl- or hydroxyalkyl-amides containing 2-8, preferably 2-6 carbon atoms in the molecule, f) heterocyclic compounds, optionally containing hydroxyl groups, with a maximum of 8 carbon atoms in the molecule with oxygen and/or nitrogen heteroatoms, g) dialkyl sulphoxides with 1 -4 carbon atoms in the alkyl residues, however, when a compound from groups Aa) or Ab), or from groups Aa) or Ac) is present, only compounds from group De) or Df) should be added; and in a fourth step, a concentrated aqueous solution of pure E. proteases and/or amylases is added to the solution thus obtained, with stirring, in amounts such that the stability of the stabilized enzyme preparation is in the range of 1,000 to 800,000, preferably 10,000-800,000 PE/cc and/or 10-10,000, preferably 200-10,000 SKBE/cc. (Machine-translation by Google Translate, not legally binding)