Process for determining the concentration of inorganic phosphates in human fluids

摘要

In a testing procedure for determining the concentration of a organic phosphate in human fluids by determining the amount of light absorbance of a chromogen in a test sample formed by reacting the phosphate with at least two reagents in two separate steps, a true blank is prepared having a light absorbance representative of all the constituents in the sample other than the chromogen. The true blank is prepared by adding the same reagents to a blank sample in a sequence different from that used for the test sample so that the conditions under which the chromogen is formed are avoided in the blank sample.