Use of laser speckle patterns for measurement of electrophoretic mobilities

摘要

Reduction in electrophoretic mobility of antigen-coated microscopic polystyrene spheres due to an antigen-antibody reaction is detected by observing changes in oscillation amplitude of speckle patterns (i.e., regions of light and dark) produced by laser light scattered by the particles as they are driven back and forth by a square wave electric field.