Microbiological assay of aminoglycoside antibiotics

摘要

A method of measuring the level of an aminoglycoside antibiotic, such as gentamicin, tobramycin or amikacin, in blood serum or plasma comprising incubating an aliquot of blood serum or plasma from a human patient being treated with an aminoglycoside antibiotic in a urea- containing culture medium with a susceptible strain of an adaptive urease producing microorganism corresponding to Proteus rettgeri JLI 03, ATCC No. 31168.