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Luminescent conjugates for immunological analysis - comprising a complex formed between a pharmacologically, immunologically, or biochemically active ligand and a chemiluminescent cpd.
Luminescent conjugates for immunological analysis - comprising a complex formed between a pharmacologically, immunologically, or biochemically active ligand and a chemiluminescent cpd.
The ligand is a drug, an amino acid polypeptide or protein a steroid, intamin A, D, E or K, an antibiotic, a nucleic acid, a polynucleotide, a nucleoside, a saccharide, serotonin, spermine, galactose, phenyltartaric acid, pesticide fungicide or nematocide, a cell or cell extract, or a virus or virus particle. The chemiluminescent cpd. is tetrakis(dimethylamino) ethylene, luciferin, lucigenin, oxalyl chloride or a 2,3-dihydrophthalazine-1,4-dione of formula (I) (where R is NH2, NHMe, NHEt, NHCH2-Ph, NHCOCH2Cl, NHCOCH2NH2NHCOCH -2NHNH2, Nme2 Net2 or N(CH2Ph)2). The conjugates can be used as chemiluminescent-labeled antigens for the immunological determination of the corresp antigen (ligand) in body fluids by the competitive binding method. Antigen concns. as low as 5-25 ng/cm3 can be determined. In an example, an insulin-luminol conjugate is used to determine the insulin content of blood plasma.
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机译:配体是药物,氨基酸多肽或蛋白质,类固醇,intamin A,D,E或K,抗生素,核酸,多核苷酸,核苷,糖,5-羟色胺,精胺,半乳糖,苯基酒石酸,农药杀菌剂或杀线虫剂,细胞或细胞提取物或病毒或病毒颗粒。化学发光的cpd。是四(二甲氨基)乙烯,萤光素,荧光素,草酰氯或式(I)的2,3-二氢酞嗪-1,4-二酮(其中R为NH2,NHMe,NHEt,NHCH2-Ph,NHCOCH2Cl,NHCOCH2NH2NHCOCH -2NHNH2 ,Nme2 Net2或N(CH2Ph)2)。缀合物可以用作化学发光标记的抗原,通过竞争结合方法对体液中的corresp抗原(配体)进行免疫学测定。抗原浓缩物。可以确定低至5-25 ng / cm3。在一个实例中,胰岛素-鲁米诺缀合物用于确定血浆中的胰岛素含量。
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