LATEX REAGENT SENSITIZED WITH HEMOLYTIC STREPTOCOCCAL NICOTINAMIDE ADENINE DINUCLEOTIDASE *NADASE** METHOD OF PRODUCING THE SAME AND METHOD OF DETECTING NADASE ANTIBODY USING THE SAME

摘要

PURPOSE:To produce the subject reagent capable of detecting a nicotinamide adenine dinucleotidase (NADase) antibody with high sensitivity, conveiniently and easily and at cheap cost, by sensitizing latex particles with hemolytic streptococcal NADase. CONSTITUTION:Particles of synthetic high-molecular latex are sensitized with NADase purified from a hemolytic streptococcal cultivation filtrate to produce a sensitized latex reagent. In the sensitization, latex particles are brought into contact with the antigen in an aqueous medium such as water, or a physiological saline solution, and left standstill, agitated or shaked. After the sensitization, the latex particles are washed with the aqueous medium to eliminate surplus antigen. The thus obtained latex reagent is stable, but it can be preserved for a long period of time by, for example, freeze-drying. The freeze-dried article is dissolved by adding a diluent, when used.