Enhancement of the sensitivity of viable microbial cell count by bioluminescent assay of ATP and its use for identification of microbes by means of a short incubation in nutrient media

摘要

A method of increasing the sensitivity and accuracy of the measurement of viable microbial cells by the bioluminescent assay of ATP by means of activating the metabolism of inactive viable cells and by increasing the number of microbial cells or biomass during a short incubation period in a microbiological nutrient medium. The utilization of a short incubation of the sample in specific nutrient media that support or prevent the growth of particular species of microbes allows the detection and identification of specific species in a few hours.