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Process for obtaining new strains of yeast for bread-making and novel strains of yeast thus prepared

机译:获得用于制面包的新酵母菌株的方法和由此制备的新酵母菌株

摘要

A complete and reproducible process for producing novel strains of yeast, comprises making a first screening test and at least one other screening test selected from a group of screening tests which do not resort to any measurement of gas release, selecting by means of said first and at least one other said screening test the desired strains from a group of diploid strains prepared previously either by hybridation, or by mutation of existing strains. The tests are as follows: A first test consists of measuring the average multiplication coefficient of a given strain by following the optical density variation of a standard medium seeded by a suspension of cells obtained from this strain. A second test consists of measuring in the same manner the average multiplication coefficient of the said strain in the presence of an inhibitor acid added to the standard medium. A third test consists of measuring the maltose adaptation of said strain in the presence of glucose by determining the amount of maltose subsisting in a standard medium after a known amount of glucose added to this medium has been completely consumed. A fourth test consists of measuring the invertase content of said strain. A fifth test consists of measuring the latent time of said strain. The hybridation can consist of systematic haploid crossings derived from quick Saccharomyces Cerevisiae strains adapted to maltose and haploids derived from very slow strains not adapted to maltose, but well adapted to sweet doughs and sometimes also to acid doughs.
机译:一种完整且可再现的生产新型酵母菌株的方法,包括进行第一筛选试验和至少一个其他筛选试验,这些筛选试验选自不进行气体释放量测量的一组筛选试验,通过所述第一和第二种方法进行选择。至少另一个所述筛选测试从先前通过杂交或通过现有菌株的突变制备的二倍体菌株的组中期望的菌株。测试如下:第一测试包括通过遵循由该菌株获得的细胞悬浮液接种的标准培养基的光密度变化来测量给定菌株的平均增殖系数。第二测试包括在添加到标准培养基中的抑制剂酸存在下,以相同的方式测量所述菌株的平均增殖系数。第三项测试包括在葡萄糖存在下,通过确定完全添加到该培养基中的已知葡萄糖量后,确定存在于标准培养基中的麦芽糖量来测量所述菌株对麦芽糖的适应性。第四项测试包括测量所述菌株的转化酶含量。第五测试包括测量所述应变的潜伏时间。杂交可以由衍生自适于麦芽糖的快速酿酒酵母菌株的系统性单倍体杂交和衍生自不适应于麦芽糖,但非常适应甜面团以及有时还适应酸性面团的非常慢的菌株的单倍体。

著录项

  • 公开/公告号US4318929A

    专利类型

  • 公开/公告日1982-03-09

    原文格式PDF

  • 申请/专利权人 LESAFFRE ET CIE;

    申请/专利号US19770863952

  • 发明设计人 ANNIE LOIEZ NEE HENNETTE;PHILIPPE CLEMENT;

    申请日1977-12-23

  • 分类号A23L1/28;C12N1/18;

  • 国家 US

  • 入库时间 2022-08-22 12:14:03

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