SELECTIVE HIGH-SENSITIVE DETECTION METHOD OF STEROID HORMONE INTERMEDIATE

摘要

PURPOSE:To detect steroid hormone intermediates having a hydroxyl group at the C3 position economically, easily, quickly, in high sensitivity, and accurately. CONSTITUTION:Various kinds of steroid hormone intermediates having a hydroxl group at the C3 position in a specimen are separated into each of components using preferably high-speed liquid chromatography, an elute containing each intermediate component separated successively is mixed with a reaction solution containing nicotinamide adenine nucleotide, the prepared mixed solution is then passed through an immobilized enzyme column maintaining 3alpha-hydroxy steroid dehydrogenase and/or 3beta-hydroxy steroid dehydrogenase, so that each intermediate component is successively reacted with nicotinamide adenine nucleotide. Each steroid hormone intermediate component is detected by measuring the fluorescence of a fluorescent substance obtained by the reaction.