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METHOD FOR CONVERTING ADENOSINE MONOPHOSPHATE INTO ADENOSINE TRIPHOSPHATE

机译:将腺苷单磷酸转化为三磷酸腺苷的方法

摘要

PURPOSE:To obtain adenosine triphosphate (ATP) in high yield in a short time stably for a long period, by treating adenosine monophosphate (AMP) with an immobilized enzyme capable of converting AMP produced by a microorganism having a relatively high optimum growth temperature and an immobilized enzyme capable of converting adenosine diphosphate (ADP) into ATP in a specific concentration. CONSTITUTION:An enzyme capable of converting adenosine monophosphate (AMP) produced by a microorganism, e.g. Bacillus stearothermophilus, having 50-85 deg.C optimum growth temperature into adenosine diphosphate (ADP) or an enzyme capable of converting ADP into adenosine triphosphate (ATP) is immobilized to give an immobilized enzyme capable of converting AMP into ADP and ADP into ATP. The resultant immobilized enzymes are combined, and the concentration of AMP is set at =5mM to treat AMP. Thus, AMP is converted into ATP.
机译:目的:通过用固定化酶处理单磷酸腺苷(AMP),该酶能够转化由具有相对较高最佳生长温度的微生物产生的AMP,并在短时间内稳定,高产量地获得三磷酸腺苷(ATP)。能够将特定浓度的二磷酸腺苷(ADP)转化为ATP的固定化酶。组成:一种能够转化微生物产生的单磷酸腺苷(AMP)的酶。将具有50-85℃的最佳生长温度的嗜热脂肪芽孢杆菌转化为二磷酸腺苷(ADP)或将ADP转化为三磷酸腺苷(ATP)的酶进行固定,从而获得一种固定化的酶,该酶能够将AMP转化为ADP和将ADP转化为ATP。合并所得的固定化酶,并将AMP的浓度设置为≥5mM以治疗AMP。因此,AMP被转换为ATP。

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