THIN TUBE TYPE ISOTACHOPHORESIS ANALYSIS

摘要

PURPOSE:To prevent the formation of a mixed zone of both liquids by monitoring the introduction of a terminal liquid and a leading liquid introduced thereafter at an exhausting hole provided deviatedly to an electrophoresis tube. CONSTITUTION:The terminal electrolyte T is introduced from a terminal liquid electrode cell 2. Since the exhausting hole 7 provided deviatedly to the electrophoresis tube 3 is opened, the electrolyte T is exhausted from the hole 7 in order, and the terminal electrolyte introduction is stopped when the exhausting is attained. Next, the leading electrolyte L is introduced from a leading liquid electrode cell 4, said electrolyte is attained to the hole 7 and exhausted partially together with the terminal electrolyte. At the exhausting time point, the hole 7 is closed by an on-off valve 9. Thereby, a boundary surface P of both electrolytes is formed clearly. The sample is injected from a sample injecting hole 6 by using a microsyringe 11. Since a needle 12 of the microsyringe 11 is not passed through the terminal electrolyte at all, the mixed zone of both electrolytes is not formed.