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FACTOR VIII CONCENTRATES PREPARED FROM HEPARINIZED PLASMA BY THE APPLICATION OF A COLD PRECIPITATION TECHNIQUE
FACTOR VIII CONCENTRATES PREPARED FROM HEPARINIZED PLASMA BY THE APPLICATION OF A COLD PRECIPITATION TECHNIQUE
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机译:应用冷沉淀法从肝素化血浆中提取的凝血因子浓缩物
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摘要
ABSTRACT OF THE DISCLOSUREA method for recovering Antihemophilic Factor VIII from wholeblood or blood plasma or blood plasma fractions freshly collected directlyinto an anticoagulant selected from the group of heparin, sodium heparin,or mixtures thereof, the heparin being used in the range of 6 - 8 units/mlof plasma or 3.5 - 4 units/ml of blood; and either first separating the redcells from the plasma and/or freezing the heparinized plasma,resolubilizing the plasma, isolating a cryoprecipitate from the plasma,resolubilizing the cryoprecipitate, adding a saline heparin solution to theresolubilized cryoprecipitate, incubating the resolubilized cryoprecipitateat a temperature of from a out 0°C to about 4°C for a time of at least2 hours, whereby Factor VIII present in the cryoprecipitate isinsolubilized using heparin precipitable cold insoluble globulin and theresulting Factor VIII rich precipitate also includes cold insolubleglobulin, separating the Factor VIII rich precipitate, and isolating FactorVIII therefrom. This new process permits recovery of a high purity FactorVIII preparation which can easily be made in the blood bank oralternatively, the cold precipitation step can be carries out in afractionation plant.
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