ANALYZING METHOD OF SAMPLE COMPONENT BY HIGH-SPEED LIQUID CHROMATOGRAPHY USING ABSORBANCE DETECTOR

摘要

PURPOSE:To identify easily a sample by detecting the absorbance with the light of a wavelength at which an eluate indicates the absorbance of a specified level as a detection wavelength and obtaining a chromatogram indicating the difference of the absorbance between the eluate and sample component. CONSTITUTION:An eluate tank 1, a pipe 2, a liquid feed pump 3, a sample injector 4, a column 5, an absorbance detector 6, a waste liquid tank 7 and a recorder 8 are provided. The eluate lifted from the tank 1 by the pump 3 is discharged through the pipe 2 to the tank 7. The sample is injected from the injector 4 into the eluate during the course of said discharge and is subjected to sepn. and elution in the elution time intrinsic to components by the interaction in the stage of passing through the column 5 and passes through the detector 6. The detector 6 detects the change of the absorbance of the eluate with time and applies the same to the recorder 8 so that the change is recorded by the recorder 8. The simultaneous analysis of the sample is thus made possible.