chromatography binder, methods of preparing a separation matrix, fabricating a chromatography column and separation membrane, and separating one or more antibodies from one or more other compounds in a liquid sample, separation matrix , use thereof, antibody purification kit for one or more other components in a liquid, and disposable chromatography column

摘要

BINDING CHROMATOGRAPHY, METHODS FOR PREPARATION OF A SEPARATION MATRIX, MANUFACTURE OF A CHROMATOGRAPH COLUMN, AND A SEPARATION MEMBRANE, AND SEPARATION OF ONE OR MORE SEPARATE COMPOSITIONS , USE OF THE SAME, KIT FOR PURIFICATION OF ANTIBODIES OF ONE OR MORE COMPONENTS IN A LIQUID, AND, DISPOSABLE CHROMATOGRAPH COLUMN. The present invention relates to a chromatography binder defined by the following formula (I) R 1 -R 2 R -N-N (R 3 R) 4 R-5 R 5 wherein R is Is a substituted or unsubstituted phenyl group; R2 is a hydrocarbon chain comprising from 0 to 4 carbon atoms; R3 is a hydrocarbon chain comprising from 1 to 3 carbon atoms; R4 is a hydrocarbon chain comprising from 1 to 5 carbon atoms; and R 5 is OH or H. The invention also comprises a separation matrix comprising the described ligands coupled to a porous support such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is antibody purification.