chromatography binder, methods of preparing a separation matrix, and separating one or more antibodies from one or more other compounds into a liquid sample, separation matrix, and disposable chromatography column.

摘要

chromatography binder, methods of preparing a separation matrix, manufacturing a chromatography column and separation membrane, and separating one or more antibodies from one or more other compounds in a liquid sample, separation matrix , use thereof, kit for antibody purification of one or more other components in a liquid, and disposable chromatography column. The present invention relates to a chromatography binder defined by the following formula (i): R1-R2-2-N (R3-) R4- R5, wherein R R1 is a substituted or unsubstituted phenyl group; R2 is a hydrocarbon chain comprising from 0 to 4 carbon atoms; R3 is a hydrocarbon chain comprising from 1 to 3 carbon atoms; R 4 is a hydrocarbon chain comprising from 1 to 5 carbon atoms; and r ~ 5 ~ is oh or h. The invention also comprises a separation matrix comprising the described ligands coupled to a porous support such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is antibody purification.