Hybrid cells that produce a characteristic antigen of hepatitis B virus, obtained from hepatocytes, and cells that are obtained from the monkeys, process for obtaining of hybrid cells and using them for production of the antigen

摘要

1. A process for producing a cellular hybrid expressing or susceptible of being rendered capable of expressing a sequence of cloned DNA, corresponding to at least part of the genome of the hepatitis B virus, characterized : - in that a co-culture is carried out, under conditions appropriate to allow their hybridization, on the one hand, of established eukaryotic cells of monkey, in which the aforesaid cloned DNA sequence is expressible or expressed and, on the other hand, monkey hepatocytes, preferably from the chimpanzee, this co-culture being carried out in a medium which does not permit the development of the said established cells when they are not complemented by an appropriate genetic sequence, and - in that the hybrid cells formed are collected.