A method for producing a heterologous protein Mature.

摘要

Method for producing a heterologous protein Mature.Includes Transforming a host with a recombinant vector to express effectively in a DNA Sequence that encodes the mature recombinant protein in a heterologous host, where the Vector comprises the frequency of DNA in Union with operable control sequences compatible with the host; Transformed the recombinant host and grow in an environment that maintains growth.Where the Vector also includes a Terminator sequence operably coupled to the Yeast 3 'end of the coding region, also comprising a expression vector in which the control sequences compatible with the host in a 5 to 3 direction include a promoter sequence Yeast, a sequence of signal of cellulase,And the coding region of the heterologous protein operably coupled to the signal sequence.