A procedure to produce a complementary DNA clonadoo its modified DNA that encodes a polypeptide of factor denecrosis Tumors

摘要

Procedure to produce a cloned complementary DNA or modified DNA that encodes a polypeptide of tumor necrosis factor.Includes: (a) cultivating Rabbit macrophages with inducers; b) Separating a fraction containing a Messenger RNA (mRNA) of Rabbit Tumor Necrosis Factor of cultivation of macrophages; c) treating the mRNA with reverse transcriptase.For a Single Stranded complementary DNA (cDNA); (d) to convert the Single Stranded cDNA in a double stranded cDNA; (e) The Double stranded cDNA INSERT IN Plasmid vector; (f) to introduce recombinant Plasmid in a host to transform and build a double stranded cDNA Cdna library; and G hybridize to cdna library to obtain cDNA encoding factorRabbit tumor necrosis.