DETERMINATION OF BASE SEQUENCE OF NUCLEIC ACID BY CHEMILUMINESCENCE DETECTION

摘要

PURPOSE:To achieve a measurement with an inexpensive device and with a high S/N ratio, by a method wherein a nucleic acid fragment labeled by a chemiluminescent substrate is eluted by electrophoresis, a reagent for emission of light is made to react with an elution sample to detect chemiluminescence optically. CONSTITUTION:A DNA fragment labeled by N-(4-aminobutyl)-N-ethylisoluminol or the like is injected at one end of migration columns 2-1-2-4 and an electrophoresis is performed by a phoretic voltage from a power source 18. Bands (a), (b)... of the DNA fragment eluted from the other end of the migration columns 2-1-2-4 are carried through a passage 10 by a buffer 14. Then, hydrogen peroxide, potassium ferricyanide and caustic soda are mixed sequentially and then, a solution is introduced into a flowcell 16 to detect chemiluminescence. The results of detection are processed by a signal processing section 34 to determine the sequence of DNA.