NUCLEIC ACID DETECTION METHOD, NUCLEIC ACID QUANTITATIVE DETERMINATION METHOD, NUCLEIC ACID BASE SEQUENCE IDENTIFICATION METHOD, NUCLEIC ACID MUTATION OR POLYMORPHISM IDENTIFICATION METHOD, NUCLEIC ACID DETECTION KIT, AND REACTION CHIP

摘要

The present invention relates to a method for detecting a target nucleic acid using a catalytic nucleic acid, which can improve the reaction efficiency of a catalytic nucleic acid and achieve continuous reaction under isothermal conditions. The method for detecting a target nucleic acid includes a reaction step to obtain a first nucleic acid product by hybridizing a first nucleic acid which is a substrate to a first binding site of a catalytic nucleic acid equipped with an active site having a catalytic action; and a detection step for detecting at least one of the first nucleic acid product or unreacted first nucleic acids, wherein, the hybridization is carried out in the presence of a cationic comb-type polymer.