BT INSECTICIDAL PROTEIN GENE AND PRODUCTION OF LARGE AMOUNT OF BT INSECTICIDAL PROTEIN USING SAME

摘要

PURPOSE:To produce a large amount of insecticidal protein, by preparing a manifestation plasmid containing an insecticidal protein gene of Bacillus thuringiensis Aizawa a7A021 strain and capable of manifesting said gene in a microbial cell. CONSTITUTION:A manifestation vector capable of producing an insecticidal protein of Bacillus turningiensis Aizawa a7A021 strain in a microbial cell such as E.coli, etc., is prepared by linking an insecticidal protein gene of Bacillus thuringen Aizawa a7A021 strain to a manifestation vector containing Lac promoter such as manifestation vector pUC18, etc. The obtained manifestation vector is introduced into a host microorganism such as E.coli and the obtained transformed microorganism is cultured under proper condition to obtain a large amount of insecticidal protein.