METHOD OF PROTEIN DETERMINATION BY MEANS OF DETECTION PRACTICE

• 1. 肝型脂肪酸结合蛋白质制剂、对其评价的方法、抑制使用其的测定中的肝型脂肪酸结合蛋白质引起的测定值的变动幅度的方法、肝型脂肪酸结合蛋白质、其的制造方法、编码其的DNA、由该DNA转化得到的细胞、制作肝型脂肪酸结合蛋白质的校准曲线的方法及对该蛋白质进行定量的方法 [P] . 中国专利： CN109121419A . 2019-01-01
• 2. 蛋白质测定方法、蛋白质测定用指示剂及蛋白质测定用试验片 [P] . 中国专利： CN100387992C . 2008.05.14
• 3. SET FOR PROTEINS DETERMINATION BY MEANS OF DETECTION PRACTICE WITH SILVER APPLICATION [P] . CS259361B1 . 1988-10-14

  机译： 结合银的检测方法测定蛋白质的装置

• 4. Method by practicing protein determination detection [P] . CS739385A1 . 1988-03-15

  机译： 进行蛋白质测定检测的方法

• 5. Determination of the modified lipoprotein, a reagent for the determination of the modified lipoprotein, detection reagent of cardiovascular disease detection methods and of cardiovascular disease [P] . 日本专利： JPWO2003006989A1 . 2004-11-04

  机译： 修饰脂蛋白的测定，修饰脂蛋白的测定试剂，心血管疾病的检测方法及心血管疾病的检测试剂

• 1. Determination of Fructose and Glucose Contents in Honey by Liquid Chromatography-refractive Index Detection Method and Direct Titration Method [J] . Xiaoya PU, Xusheng DU, Bo LIU, 农业生物技术：英文版 . 2016,第002期
• 2. A rapid method for the determination of dopamine in porcine muscle by pre-column derivatization and HPLC with fluorescence detection [J] . Hong-Xia Zhao, Hui Mu, Yan-Hong Bai, 西安医科大学学报(英文版) . 2011,第003期
• 3. A rapid method for the determination of dopamine in porcine muscle by pre-column derivatization and HPLC with fluorescence detection [J] . Hong-Xia Zhao, Hui Mu, Yan-Hong Bai, 药物分析学报（英文） . 2011,第003期
• 4. Fundamentals of the ASTM International Committee D22 Practice for Determination of the Detection Limit of a Weil-Behaved Analytical Method [C] . Michael J. Brisson, Harry L. Rook Symposium on detection limits in air quality and environmental measurements . 2019
• 5. Methods Development for the Fluorescent Determination of Inorganic Phosphate and Phospho-Proteins [D] . Awada, Angela. 2020
• 6. Evaporative Light Scattering Detection Based HPLC Method for the Determination of Polysorbate 80 in Therapeutic Protein Formulations [O] . Vikram S. Nayak, Zhijun Tan, Peter M. Ihnat, -1
• 7. Analysis of multiple protein detection methods in human osteoporotic bone extracellular matrix: From literature to practice [O] . Caterina Licini, Giorgia Montalbano, Gabriela Ciapetti, 2020
• 8. Determination of L-Fucose in Glycoproteins. II. An Automated Method for the Determination of L-Fucose in Serum Glycoprotein Hydrolysates [R] . Sobocinski, P. Z., Canterbury, W. J., Hartley, K. M. 1972

• 1. Angstrom-technology： Detection of proteins on cancer cells could play a role in the methods of therapy [J] . Ma＇an Nassar Raja AL-Ani ,Martin C. Richardson . 材料科学与工程：中英文版 . 2009,第012期
• 2. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 3. 对GB/T5511-2008《谷物和豆类氮含量测定和粗蛋白质含量计算凯氏法》检测方法的探讨 [C] . 周勋 . 第七届中国粮油标准质量学术年会 . 2015
• 4. 蛋白质测定方法及小麦核心种质蛋白质含量的研究 [A] . 李冬梅 . 2006