Method for preparing prhw11 shRNA expression and prhw12

摘要

Process for the preparation of prhw11 and prhw12 shRNA expression.Wherein the larger Fragment of Plasmid prhw10 is contained in the per103 HindIII Fragment of Plasmid DNA of General formula (i); for the preparation of Plasmid prhw12 uses The Clone p9a2; because for the preparation of Plasmid phrw11 uses the clone E76e9; and because the Plasmid is inserted in the place per103, HindIII,Through reaction with ligase, the DNA fragment of General formula (i). Application in Medicine.