Imaging immunoassay detection system and method

摘要

@ Light generated by one ormore chemical reactions in a discrete array is determined, both as to emissions per reaction and as to relative location of the reactions, e.g. using a system comprising:a sample carrier having spaced-apart discrete areas each containing a reactant capable of emitting light photons if a reaction takes place;a photon receiver, for receiving photons emitted from each reaction simultaneously; anda signal generator coupled to the photon receiver, for generating a signal representing the x-y location of each reaction.;The signal generator may be a photomultiplier, charge-coupled device or vidicon tube. The invention is particularly suited to the determination of reactions and non-reactions, e.g. between an array of labelled antibodies and a specimen potentially containing one or more complementary antigens, in an immunoassay.