glukosisomerasgen, recombinant dna innehaallande glukosisomerasgenen and microorganism transformed with the recombinant dna naemnda

摘要

PURPOSE:To enable mass-production of glucose isomerase, by separating a gene coding a glucose isomerase originated from actinomycetes and clarifying the structure of the gene. CONSTITUTION:A polymeric DNA of an actinomycete cell is completely digested with a restriction enzyme Bam H1 and a DNA fragment fraction having a chain length of about 5.7kb is separated by neutral sucrose density-gradient centrifugal separation process. The fragment is linked to Bam H1 site of a pUC vector using a ligase and transformed to E.coli to obtain a DNA library. The glucose isomerase gene is selected from the library by using a probe consisting of an oligonucleotide labeled with 32P.