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glukosisomerasgen from streptomyces griseofuscus s 41 and microorganism transformed with the recombinant dna containing the gene
glukosisomerasgen from streptomyces griseofuscus s 41 and microorganism transformed with the recombinant dna containing the gene
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机译:链霉菌41型链霉菌的gluukosisomerasgen和含有该基因的重组DNA转化的微生物
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摘要
PURPOSE:To enable mass-production of glucose isomerase, by separating a gene coding a glucose isomerase originated from actinomycetes and clarifying the structure of the gene. CONSTITUTION:A polymeric DNA of an actinomycete cell is completely digested with a restriction enzyme Bam H1 and a DNA fragment fraction having a chain length of about 5.7kb is separated by neutral sucrose density-gradient centrifugal separation process. The fragment is linked to Bam H1 site of a pUC vector using a ligase and transformed to E.coli to obtain a DNA library. The glucose isomerase gene is selected from the library by using a probe consisting of an oligonucleotide labeled with 32P.
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