PURPOSE:To allow the analysis in real time within a short period of time with high accuracy by adjusting the pH of a culture solution to a set value, then evaporating the dissolved components in the culture solution and analyzing the evaporated dissolved components. CONSTITUTION:The supernatant of the culure solution is sampled by a sampling pump 8 and a filter device 9 of a cross flow system from the culture solution 7 and a pH control agent is dropped 10 by an electric signal. While the flow rate of gas is adjusted in an evaporating device 11, the pH is controlled and the supernatant liquid is brought into contact with the carrier gas and the dissolved components are separated from the supernatant liquid into the carrier gas. The temp. is kept 12 at a set temp. during the above-mentioned stages. The dissolved components which are evaporated are subjected to measurement 13 of the mass analysis value and the supply rate of the culture medium is determined from this value by a culture controller 14. The supply rate of the medium is thus controlled 15. Since the continuous analysis of the dissolved components of the culture solution are enabled in a short period of time with the high accuracy, the analysis in the real time is possible and the efficient culture is executed when the culture conditions are controlled in accordance with the analysis value.
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