首页>
外国专利>
METHOD FOR SELECTING CHROMOSOME-SPECIFIC REPETITIVE DNA ARRANGEMENT USING RECOMBINANT DNA CLONE
METHOD FOR SELECTING CHROMOSOME-SPECIFIC REPETITIVE DNA ARRANGEMENT USING RECOMBINANT DNA CLONE
展开▼
机译:重组DNA克隆选择染色体特异性重复DNA排列的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
A method is provided for determining specific nucleotide sequences useful in forming a probe which can identify specific chromosomes, preferably through in situ hybridization within the cell itself. In one embodiment, chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family members and consensus sequences of the repetitive DNA families for the chromosome preferential sequences. The selected low homology regions are then hybridized with chromosomes to determine those low homology regions hybridized with a specific chromosome under normal stringency conditions. In accordance with the invention, oligomers are provided which are useful in forming a probe for specific chromosome identification under normal stringency conditions.
展开▼