We introduce a homology-based recombination approach for generating a cDNA construct. This method depends on amplifying several exon fragments and their fusions by the homology-based recombination. This method provides a way to generate the cDNA sequence of any gene without any need for its mRNA. The paper describes the strategy by assembling cDNA of the MYB1 and MYB2 genes of Arabidopsis thaliana.
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机译:metodi microbiologici Tradizionali e metodi moleculeolari per l'analisi degli integratori alimentari a base di o con probiotici per ujso umano(microbiological and molecular methods for analysis of probiotic Based Food supplements for Human Consumption)。