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NOVEL PORCINE CHORELA ATTENUATED VIRUS STRAIN, PREPARATION THEREOF, PORCINE LIVE VACCINE AND ITS PRODUCTION
NOVEL PORCINE CHORELA ATTENUATED VIRUS STRAIN, PREPARATION THEREOF, PORCINE LIVE VACCINE AND ITS PRODUCTION
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机译:新型猪霍乱减毒病毒株,其制备,猪活疫苗及其生产
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摘要
PURPOSE:To synthesize a porcine cholera attenuated virus strain by subculturing and acclimating a lapinized porcine cholera attenuated virus LPC-China strain without passing through a primary cultured cell of an animal into an established cell derived from the porcine kidney. CONSTITUTION:A lapinized porcine cholera attenuated virus LPC-China strain is subcultured and acclimated into an established cell derived from the porcine kidney, such as an established cell originating from PK15, without passing through a primary cultured cell of an animal to prepare a porcine cholera attenuated virus strain. The LPC-China strain is preferably obtained by a method for subculturing the China strain with an MPKIIIaCl1 cell which is an established cell derived from the porcine kidney for 10 passages, cloning the established cell PK15 and further subculturing and acclimating the resultant cloned NPK cell for 10 passages. The obtained porcine cholera attenuated virus strain can be used to efficiently produce an excellent porcine cholera vaccine.
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