Novel porcine cholera attenuated virus strain, production method thereof, live porcine cholera vaccine and production method thereof

摘要

PURPOSE:To synthesize a porcine cholera attenuated virus strain by subculturing and acclimating a lapinized porcine cholera attenuated virus LPC-China strain without passing through a primary cultured cell of an animal into an established cell derived from the porcine kidney. CONSTITUTION:A lapinized porcine cholera attenuated virus LPC-China strain is subcultured and acclimated into an established cell derived from the porcine kidney, such as an established cell originating from PK15, without passing through a primary cultured cell of an animal to prepare a porcine cholera attenuated virus strain. The LPC-China strain is preferably obtained by a method for subculturing the China strain with an MPKIIIaCl1 cell which is an established cell derived from the porcine kidney for 10 passages, cloning the established cell PK15 and further subculturing and acclimating the resultant cloned NPK cell for 10 passages. The obtained porcine cholera attenuated virus strain can be used to efficiently produce an excellent porcine cholera vaccine.