FUSED OXIDASE OF CYTOCHROME P450C21 AND YEAST NADPH-CYTOCHROME P450 REDUCTASE, GENE CAPABLE OF CODING THE SAME ENZYME AND PRODUCTION OF THE SAME ENZYME

摘要

PURPOSE:To enhance efficiency of electron transport and improve activity per P450 molecule by preparing a fused enzyme having P450c21 on the N- terminal side and a yeast reductase on the C-terminal side. CONSTITUTION:A yeast reductase gene is connected to the 3'-terminal side of a bovine adrenal cytochrome P450c21 gene to provide a single gene and a fuse enzymic gene capable of coding an oxidase having both one atom oxygenation activity of P450c21 and reductive force supplying ability from NADPH (reduced from nicotinamide adenine dinucleotide phosphate) of NADPH-P450 reductase in the same molecule is constructed and introduced into an intrayeast expression vector and construct an expression plasmid.