FUSED OXIDASE OF CYTOCHROME P450C AND YEAST NADPH-CYTOCHROME PE450 REDUCTASE, GANE ENCODING THE SAME ENZYME AND PRODUCTION OF THE ENZYME

摘要

PURPOSE: To obtain a gene useful for producing acetophenone. ;CONSTITUTION: A fused gene consists of a DNA comprising a sequence encoding a range participating in microsome membrane bond of microsome type protein, a range sequence encoding participating at least in a substrate bond of rat liver cytochrome P450c and a range participating in heme bond and a DNA encoding a range participating at least in flavin mononucleotide and flavin adenine dinucleotide bond of yeast reductase enzyme gene bonded to its 3' end, encodes a fused oxidase having both one atom oxygen addition activity of rat liver cytochrome P450 and reducing force supplying ability of yeast NADPH-cytochrome P450 reductase and can be constituted by connecting both the DNA.;COPYRIGHT: (C)1994,JPO&Japio