PROCESS FOR PREPARING FINE DISTRIBUTED MICROPARTICLES OF TYROSINE, IN WHICH AN ALLERGEN IS DISPERSED.

摘要

1492973 Modified allergens BEECHAM GROUP Ltd 5 Feb 1975 [16 Feb 1974] 7156/74 Addition to 1377074 Heading A5B Finely divided micro-particles of tyrosine having a water soluble dialdehyde - treated allergen dispersed therein are prepared by mixing an aqueous solution of a water - soluble dialdehyde e.g. glutaraldehyde modified allergenic extract of an allergenic material with a solution of tyrosine in a strong aqueous acid e.g. hydrochloric acid; and simultaneously or subsequently neutralising (i.e. adjusting the pH to from 4 to 7.5) such mixture of solutions thereby precipitating the required product. Sodium hydroxide may be employed to neutralise the reaction mixture. The aqueous solution of modified allergen may be obtained directly by reacting an aqueous solution of an extract of allergenic material with a dialdehyde under neutral conditions. The allergen may be an extract of one or more pollens e.g. grass or ragweed pollen; house dust, the house dust mite Dermatophagoides pteronyssinus, moulds, animal hair, dander or fur. The precipitate of tyrosine containing the dialdehyde treated allergen may be removed from the solution by centrifugation or filtration and washed before being resuspended in a physiologically acceptable carrier such as phenol-saline or sterile water, to produce an injectable composition for use in desensitisation therapy.