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A METHOD FOR THE CONVERSION OF A CELLULOSIC SUBSTRATE TO GLUCOSE USING MICROBISPORA BISPORA, STRAIN RUTGERS Pamp;W
A METHOD FOR THE CONVERSION OF A CELLULOSIC SUBSTRATE TO GLUCOSE USING MICROBISPORA BISPORA, STRAIN RUTGERS Pamp;W
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机译:利用微双歧杆菌,拉丝酵母P&W将纤维素基质转化为葡萄糖的方法
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摘要
A method is described for the enzymatic saccharification of a cellulosic substrate to glucose which utilizes fermentation of the substrate with the cellulase enzyme complex-producing, thermophilic microorganism Microbispora bispora Rutgers P&W and its mutants. The resistance of M. bispora Rutgers P&W cellulase to end-product inhibition enables conversion efficiency superior to that of known cellulase producing microorganisms. Continued enzyme activity at elevated temperatures allows broader applicability of enzyme-catalyzed saccharification than heretofore attained. The microorganism in its purified form incubated on cellulase, and its cellulolytic mutants, are also disclosed.
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机译:描述了将纤维素底物酶促糖化为葡萄糖的方法,该方法利用底物与产生纤维素酶复合物的嗜热微生物Microbispora bispora Rutgers P&W及其突变体的发酵。 M. bispora Rutgers P&W纤维素酶对终产物抑制的抗性使转化效率优于已知的产生纤维素酶的微生物。在高温下持续的酶活性使酶催化的糖化作用比迄今为止获得的更广泛的应用。还公开了在纤维素酶上温育的纯化形式的微生物及其纤维素分解突变体。
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