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Detection of avian and mammalian retrovirus infections

机译:禽和哺乳动物逆转录病毒感染的检测

摘要

The invention relates to a method of detecting a retroviral pol gene product in a sample, which comprises: (a) treating the sample with a disrupting agent to liberate the pol gene product, (b) contacting the treated sample of step (a) with a pol gene product-specific monoclonal antibody, to form a pol gene product/pol gene product-specific monoclonal antibody complex, (c) separating the complex of step (b) from the sample, and (d) determining the presence of the pol gene product either in the complex described in step (b) or after separation described in step (c).
机译:本发明涉及一种检测样品中逆转录病毒pol基因产物的方法,该方法包括:(a)用破坏剂处理样品以释放pol基因产物,(b)使步骤(a)的处理过的样品与pol基因产物特异性单克隆抗体,形成pol基因产物/ pol基因产物特异性单克隆抗体复合物,(c)从样品中分离步骤(b)的复合物,和(d)确定pol的存在步骤(b)所述复合物中或步骤(c)所述分离后的基因产物。

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