this. Fusion proteins containing outer membrane protein A of E. coli and Gram-negative bacteria and methods for their expression

摘要

The invention relates to fusion proteins with the outer membrane protein A (OmpA) of Gram-negative bacteria and to processes for the expression of homologous and heterologous proteins on the outer membrane of E.coli and other Gram-negative bacteria. This entails the proteins to be expressed being inserted in the form of their cDNA into the outer membrane protein A (OmpA) in the region of the codons of one of the four outer vertices of this protein. Furthermore, these proteins can be inserted there additionally into a "stalk" which is formed by the amino terminus and carboxyl terminus of the haemagglutinin molecule from influenza virus and in turn is inserted in one of the ompA vertices. The fusion proteins are expressed in the bacteria in a controlled manner.