METHOD OF EXTRACTING 5Ъ-NUCLEOTIDASE FROM COBRA VENOM

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 to the enzyme industry, in particular to a receiving net 5 @ - u043du0443u043au043bu0435u043eu0442u0438u0434u0430u0437u044b u00a0u0434u0430 cobra - specific enzyme catalysing hydrolysis 5 @ - u0440u0438u0431u043eu043du0443u043au043bu0435u043eu0442u0438u0434u0430 to u0440u0438u0431u043eu043du0443u043au043bu0435u043eu0437u0438u0434u0430 and u043eu0440u0442u043eu0444u043eu0441u0444u0430u0442u0430. u0432u044bu0441u043eu043au0430u00a0 specific 5 - u043du0443u043au043bu0435u043eu0442u0438u0434u0430u0437u044b makes its product to study the structure of nucleic acids.purpose u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 u00a0u0432u043bu00a0u0435u0442u0441u00a0 simplification method and improving the purity of the target product by u0440u0430u0437u0434u0435u043bu0435u043du0438u00a0 5 @ - u043du0443u043au043bu0435u043eu0442u0438u0434u0430u0437u044b from u043du0435u0441u043fu0435u0446u0438u0444u0438u0447u0435u0441u043au043eu0439 u0444u043eu0441u0444u043eu043cu043eu043du043eu044du0441u0442u0435u0440u0430u0437u044b. method includes dissolving u00a0u0434u0430 cobras in u0431u0443u0444u0435u0440u043du043eu043c solution followed by u0446u0435u043du0442u0440u0438u0444u0443u0433u0438u0440u043eu0432u0430u043du0438u0435u043c and u0445u0440u043eu043cu0430u0442u043eu0433u0440u0430u0444u0438u0440u043eu0432u0430u043du0438u0435u043c on u0433u0435u043fu0442u0438u043b - u0430u0433u0430u0440u043eu0437u0435 using as 0.35 to 0.45 m buffer solution u0430u043cu043cu043eu043du0438u00a0 sulfate, ph 6.5 to 7.5.5 - u043du0443u043au043bu0435u043eu0442u0438u0434u0430u0437u0430 u044du043bu044eu0438u0440u0443u0435u0442u0441u00a0 after the principal amount of protein. exit - 90%, the degree of purification of - 25, the content of u0444u043eu0441u0444u043eu0434u0438u044du0441u0442u0435u0440u0430u0437u044b and u043du0435u0441u043fu0435u0446u0438u0444u0438u0447u0435u0441u043au043eu0439 u0444u043eu0441u0444u043eu043cu043eu043du043eu044du0441u0442u0435u0440u0430u0437u044b is less than 0.05% of 5 - u043du0443u043au043bu0435u043eu0442u0438u0434u0430u0437u044b. 1 u0437.u043f.u0444 - lu, 6 fig.