promoters for the expression of foreign genes in yeast, plasmids, which contain these promoters and their use for the production of polypeptides.

摘要

Promoters capable of ensuring expression in yeast of genes coding for heterologous polypeptides are disclosed, which comprise a DNA fragment or mutants or sub-fragments thereof, said promoter comprising a definite nucleotide sequence commencing with an EcoRI site and terminated by another restriction site. Also disclosed are vector plasmids containing the promoters, transformed yeasts comprising these plasmids, and a process for preparing polypeptides and especially a 1,4- beta -N-acetylmuramidase.