Modulation of Promoter and Secretion Efficiency for Improved Heterologous GeneExpression in the Yeast 'Saccharomyces cerevisiae'

摘要

The yeast Saccharomyces cerevisiae has certain advantages as a host for theproduction of heterologous proteins. It is a eukaryote, in which the production of proteins of higher eukaryotes may be more successful than in bacterial cells. Specific posttranslational modifications of eukaryotic proteins are likely to take place in yeast. Yeast has the capacity to secrete proteins outside the cell, which offers the possibility for the production of secretory proteins. However, the rather modest intrinsic secretory capacity of S. cerevisiae is a drawback of the yeast expression system. The glycosylation pattern of yeast secretory proteins differs from that of higher eurkaryotes. Thus it is of interest to study the effects of yeast specific glycans on heterologous proteins. The promoter of the yeast alcohol dehydrogenase 1 gene (ADH1) was among the first regulatory regions used for heterologous gene expression.