Method of manufacture of structures organized of collagen, in particular of human origin, and structures organized corresponding collagen

摘要

These organised structures may be either fibres or membranes. According to this process, a sample of animal or human tissue, washed and asepticised beforehand, is ground in a saline solution to solubilise the collagen present in the said sample. A catalyst for reconstituting the collagen fibres is then added to the extract obtained, consisting of collagen fibrils in a minimum quantity and free from nonfibrillar collagen precipitate. After a maturing stage the reconstituted organised structures are separated from the aqueous medium by centrifuging, are washed, asepticised, packaged, freeze- dried and sterilised. Among the organised structures obtained the collagen fibres are in the isolated state, separated from their original cell tissue, in the form of a network (35) and ready for use. They exhibit a periodicity of transverse striations (37) of between 68 nm and 72 nm. IMAGE