Method for the detection of a polypeptide subunit in the presence of a quaternary protein containing the subunit

摘要

A method for the determination of a free protein subunit of a quaternary protein in a sample, which comprises:PP(a) contacting a sample with a first immunological binding partner which is or will be bound to a carrier, wherein the first immunological binding partner binds epitopic determinants bindable only on the free protein subunit;PP(b) incubating the components of step (a) for a period of time and under conditions sufficient to form an immune complex between the free protein subunit, the first immunological binding partner, and the carrier;PP(c) separating the carrier of step (b) from the sample;PP(d) adding to the carrier of step (c), a detectably-labeled second immunological binding partner, wherein the second immunological binding partner binds epitopic determinants bindable on both the free protein subunit and the quaternary protein; andP P(e) determining the detectably-labeled second immunological binding partner in the carrier or in liquid phase.