Recombinant plasmid for the expression of L-phenylalanine ammonia- lyase and transformed strain carrying same

摘要

A recombinant plasmid for the expression of phenylalanine ammonia- lyase (PAL) is constructed by incorporating therein a combined promoter comprising (a) the fusion promoter (the tac promoter) composed of the trp promoter minus 35 region and the lac UV-5 promoter minus 10 region and (b) the P.sub.L promoter of the lambda phage, the tac promoter and the P.sub. L promoter being connected so as to have the same directional property. This recombinant plasmid permits more efficient expression of PAL in Escherichia coli.