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Recombinant plasmid for the expression of L-phenylalanine ammonia-lyase and transformed strain carrying same
Recombinant plasmid for the expression of L-phenylalanine ammonia-lyase and transformed strain carrying same
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机译:用于表达L-苯丙氨酸氨解酶的重组质粒和携带该质粒的转化菌株
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摘要
A recombinant plasmid for the expression of phenylalanine ammonia-lyase (PAL) is constructed by incorporating therein a combined promoter comprising (a) the fusion promoter (the tac promoter) composed of the trp promoter minus 35 region and the lac UV-5 promoter minus l0 region and (b) the PL promoter of the lambda phage, the tac promoter and the PL promoter being connected so as to have the same directional property. This recombinant plasmid permits more efficient expression of PAL in Escherichia coli.
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