Method for low-temperature preservation of spermatozoa

摘要

The method resides in cooling the spermatozoa in a high thermal- diffusive powdered material that has preliminarily been cooled down to the refrigerant temperature. Cooling is carried out in a medium of a cryopreservative, containing a salt buffer, an antishock component, and a cryoprotector. The cryoprotector is taken in a concentration high enough for vitrification to develop in the spermatozoa. An example of the high thermal diffusive powdered material is alumina.